Density gradient centrifugation

/ˈdɛnsəti ˈgreɪdiənt ˌsɛntrɪfjuˈgeɪʃən/ noun

Definition

A centrifugation technique that separates particles based on their buoyant density using a gradient of increasing density medium (like sucrose or cesium chloride). Particles migrate to positions where their density matches that of the surrounding medium.

Etymology

Combines 'density gradient' (referring to the graduated density medium) with 'centrifugation.' Developed in the 1950s-60s, with cesium chloride gradients pioneered by Matthew Meselson and Franklin Stahl for their famous DNA replication experiments.

Kelly Says

Density gradient centrifugation is like having a molecular elevator where each particle automatically stops at exactly the right floor based on its density! It's so precise that it can separate DNA strands that differ by just one heavy nitrogen isotope, which was crucial for proving how DNA replicates.

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